USCIS decided not to speed up your case. Only the expedite request was turned down: the case itself was not denied.
The case continues in normal processing order. If your circumstances change, you can ask again with new evidence of the urgency.
Informational only, not legal advice. Go by the notice USCIS sent you, and confirm deadlines and options with USCIS or an immigration attorney.
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No. Only the request to speed things up was turned down. The application itself is still pending and continues in normal order.
Generally yes, if something changes or you have new evidence of the urgency. Sending the same request again with the same evidence is unlikely to change the answer.
For some forms, premium processing (Form I-907) is available for an extra fee. Otherwise the case follows normal processing times.